In present investigation immunomodulatory activity of Plumbago zylanica was assessed. Extraction was performed using petroleum ether, chloroform, acetone and ethanol. In-vitro investigation revealed that chloroform fraction showed best effect on phagocytosis. On the basis of chloroform extract was selected for further in-vivo investigation. On the basis of acute oral toxicity studies toxicity LD 50 of chloroform extract was found to be 1000 mg/kg. Effect of extract on Delayed Type Hypersensitivity (DTH) and hemaglutination titer was observed at 200 mg/kg, 100 mg/kg and 50 mg/kg. At all selected dose extract showed significant immunomodulatory activity in both of the selected models for study.
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